Bioethanol Production from Miscanthus sinensis Cellulose by Bioconversion
نویسندگان
چکیده
Introduction. Cellulose-containing parts of herbs are an excellent source alternative energy and can be used to produce biological ethanol. The present research aims at improving this fundamental promising area biotechnology. It introduces a new consortium microorganisms that saccharify while fermenting the substrate.
 Study objects methods. featured technical cellulose obtained from Miscanthus sinensis using hydrotropic delignification oxidation with pertrifluoroacetic acid. ethanol content in culture liquid was determined Agilent 7890B gas chromatograph flame ionization detector. biocompatibility strains studied by growing direct co-culture dense nutrient medium.
 Results discussion. objective create microbial for single-step production bioethanol cellulose. A set experiments cultivation conditions made it possible select optimal producers. two developed consortia required compositions media, which were varying ratio components measuring yield resulting liquid.
 Conclusion. best consisted Pichia stipites Y7124, Candida shehatae NCL3501, Kluyveromyces marxianus Y-4290, Zymomonas mobilis 113 1:1:1:1. parameters included: temperature = 35 ± 1°C, pH 5.2, time 16 1 h. most efficient medium had following composition (g/l): glucose – 5.0; peptone yeast extract 0.4; K2HPO4 1.5; (NH)2 HPO4 MgSO4 0.5.
منابع مشابه
One-pot bioethanol production from cellulose by co-culture of Acremonium cellulolyticus and Saccharomyces cerevisiae
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ژورنال
عنوان ژورنال: ??????? ? ?????????? ??????? ???????????
سال: 2021
ISSN: ['2074-9414', '2313-1748']
DOI: https://doi.org/10.21603/2074-9414-2021-2-387-394